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KMID : 0380620190510020103
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 2 p.103 ~ p.108
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Determination the optimum extraction method for saponin lancemasides in Codonopsis lanceolata
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Lee Min-Ju
Nam Joo-Hee
Um In-Seok
Kang Chang-Keun
No Il-Rae
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Abstract
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This study was conducted to select the optimal extraction method of codonopsis lanceolata saponin. To investigate the lancemasides content depending on each extraction method, various extractions were performed: reflux (methanol and butanol), hot water, as well as ultrasonic bath (40 kHz; continuous irradiation/interval irradiation) and ultrasonicator (20 kHz) extractions. From the result, the overall lancemasides content were the highest in ultrasonic bath (MeOH; continuous irradiation) extraction, followed by ultrasonic bath (water; continuous irradiation)>ultrasonic bath (MeOH; interval irradiation)>ultrasonicator (MeOH)>hot water>MeOH reflux>BuOH reflux extractions in that order. Sample drying method prior to ultrasonic bath extraction was more effective shade drying than freeze drying. Effective duration and temperature of extraction was 2 hr at 64°C. And ingredient change diverted from aster saponin Hb to lancemasides was identified by extraction condition such as extraction time and temperature.
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KEYWORD
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fraction, mass, reflex extraction, ultrasonic bath, ultrasonicator
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